Epigenomic analysis of various totipotent-like cells or “two-cell-stage-like” (2C-like) mESC subpopulations. (A) Experimental outline for sorting and analysis of 2C-like mESC subpopulations. (B) RT-qPCR on mESC subpopulations to validate successful FACS sorting. (C,D) A genome browser view depicting a broad genomic region (4Mb; C) and a zoom in D of the H3K4me3 profiles generated for the 2C-like mESC subpopulations and their controls. (E) PCA on the promoter-associated H3K4me3 signals of the various populations of cells. (+) Positive for marker; (−) negative for marker. (F) Correlation of H3K4me3 signal in promoters between Hhex, Erv4, or Zscan4c marker-positive mESCs (2C-like mESC subpopulations) and marker-negative mESCs; no differential sites were detected (FDR-adjusted P-value <0.05).
