Figure 5.
PnP-ChIP-seq allows detection of significant differences between 2i and serum mESCs. (A) PCA on peaks of H3K4me3, H3K27ac, and H3K4me1 PnP-ChIP-seq in 2i and serum mESCs. (rep) Replicate. (B) Genome browser views for PnP-ChIP-seq of loci showing a significant difference in H3K4me3, H3K27ac, or H3K4me1 between 2i and serum mESCs (boxed). (H3*peaks_all) All peaks of a hPTM detected in 2i and serum mESCs; (H3*peaks_sig) all peaks of a hPTM that are significantly increased in either 2i or serum mESCs. (C) GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of genes associated with differential H3K4me3 between 2i and serum mESCs.
