Figure 4.
PnP-ChIP-seq using small cell quantities of mESCs by the use of MNase. (A) Gene-centered genome browser view for PnP-ChIP-seq of H3K4me3, H3K4me1, H3K27ac, and H3K36me3. Profiles labeled with an asterisk were generated from a starting amount of 7500 mESCs; otherwise, 15,000 mESCs were used. (B) Overlap between de novo peak calls of PnP-ChIP-seq and bulk ChIP-seq. (C) Heatmap of merged peak set for various starting amount of sonicated chromatin for PnP-ChIP-seq of H3K4me3, H3K4me1, H3K27ac, and H3K36me3. (D) Cross-correlations of H3K4me3 PnP-ChIP-seq using tag counts of merged peak set.
