Figure 6.
Generation of PFF clones carrying gene-trap cassettes at the target loci via HIT-trapping. (A) Schematic showing PCR genotyping of the screened PFF clones produced via HIT-trapping (left) and representative genotyping results for targeted trapping of the PPARG locus (right). Lhx220/213 were donor-specific primers. Lanes in red/blue rectangles indicate positive forward/reverse insertions. (5/3J) 5′/3′ junction. (B) Sequence confirmation of gene-trap insertions at the PPARG locus. Genome sequences are shown in green. PAMs are shown in blue. Insertions or mismatches are shown in red. Deletions are shown as dashed lines. (m) Mismatches.
