Figure 1.
Derivation and example of RMC data. (A) DNA test elements from either BACs or whole-genome fragmentation are cloned downstream from or upstream of the TSS in different experiments. After transfection in one or more cell types, reporter-derived RNA and DNA are harvested and sequenced. The reads are mapped retaining strandedness and the normalized signal Reference strand signal minus the Complement strand signal (RMC) is calculated (created with BioRender.com). (B) RMC data from four cell lines are shown on a 20-kb portion of Chromosome 4 (hg38 coordinates). The arrows mark gene bodies and the direction of stable transcription.
