Full-length protein-encoding genes and their expression in the T. gondii mt genome are supported. Segments of an ONT read that can encode a full-length cytochrome gene are annotated with their SB name and shown below the schematic of each gene. Numbers below each gene schematic represent nucleotide start/stop positions of a SB on the gene. All blocks are in the forward orientation except block V in coxIII. Only a portion of SBs V, M, and T is included in the CDS. MtDNA-specific PE-150 Illumina DNA (SRR9200762) and RNA-seq (SRR6493545) reads were independently mapped to each of the cytochrome gene sequences. Red and blue lines below each gene indicate mapped Illumina paired-ends. Both ends were required to map. RT-PCR products and ONT RNA reads with full-length CDSs are shown.
