Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication

NHEJ-mediated DSB repair restores chromatin structure independent of replication. (A) Pictographs depicting local nucleosome changes over the experimental time course in the WT and dnl4Δ strains. Similar to the analysis performed in Figure 4, we precisely quantified the alterations in chromatin structure following break induction and NHEJ-mediated repair. Increasing time is plotted downward on the y-axis and the boundaries of the HO cut site are denoted by dotted blue lines. (B) The log₂-relative (to pre-induction) occupancy of the 1L (WT squares/solid line; dnl4Δ triangles/dashed line) and 3R (WT circles/solid line; dnl4Δ diamonds/dashed line) nucleosomes (denoted 1L and 3R, respectively, in Fig. 6C) are plotted over time for this experiment. (C) Model for the replication-independent nucleosome reassembly and chromatin restoration following NHEJ.