Local chromatin changes in response to a DSB at the PHO5 locus. GCOPs of WT (A), yku70Δ (B), and mre11Δ (C) strains over the experimental time course. Gene bodies are annotated above the plots in gray, with arrows indicating the direction of transcription. Dotted blue lines denote the 117-bp HO recognition site, and a predicted Sum1 protein binding site is also depicted. Similar to Figure 2, B and C, the black trace represents the 2D cross-correlation score and the blue shaded regions represent 0.5 standard deviations of nucleosome positions from the center of each identified nucleosome. The regions quantified in Figure 4 are marked by colored arrowheads for the 1L (blue) and 3R (gold) nucleosomes and the 1L-2L linker (red) region.
