Figure 5.
t-SNE visualization of clusters identified by Specter. Clusters of PBM cells were inferred from protein expression (ADT) alone (left) or from combined mRNA and protein expression (middle). In contrast to the joint clustering of both modalities, ADT-based clustering cannot discriminate CD14+ and FCGR3A+ monocytes, does not detect megakaryocytes (red), and does not allow to discriminate between CD27−DR+ and CD27−DR− subpopulations of CD4+ T cells. The simultaneous clustering of RNA and protein expresssion in CBM cells (right) additionally reveals a rare population of megakaryocytes (red).
