AP-1 subunits converge promiscuously at enhancers to potentiate transcription

Changes in AP-1 hotspot binding link to changes in gene expression. (A–C) Temporal gene expression trajectory plot showing log₂ fold change in gene expression mapped to the gains of each AP-1 binding classes in response to dex according to (A) eQTLs, (B) Activity by Contact defined by Hi-C coupled with chromatin accessibility (DNase-seq) and H3K27ac, and (C) the proximity to the nearest genes. (D–F) Temporal gene expression trajectory plot showing log₂ fold change in gene expression mapped to the losses of each AP-1 binding classes in response to dex according to (D) eQTLs, (E) Activity by Contact, and (F) the proximity to the nearest genes. (G–I) Temporal gene expression trajectory plot showing log₂ fold change in gene expression mapped to maintained AP-1 binding classes in response to dex according to (G) eQTLs, (H) Activity by Contact, and (I) the proximity to the nearest genes. P-values were calculated using a Wilcoxon rank-sum test based on changes in log₂ folds at 12 h. (**) P < 0.01, (***) P < 0.001, N.S. = P > 0.05.