Variation in proportion of somatic piRNAs in mosquito strains correlates with persistent arboviral small RNAs. (A) Small RNA size distributions from mosquito samples in which the somatic piRNA levels are much lower in comparison to the gonads, and these samples lack other arbovirus small RNAs. Colored lines at the bottom mark the siRNAs and miRNAs ranging between 19 and 23 nt, whereas piRNAs are between 24 and 30 nt. The inset charts magnify the distribution of transposon and virus sRNAs under a different y-axis range, and the red arrow points to low levels of somatic piRNAs. (B) Additional small RNA size distributions (left) of mosquito samples with high levels of somatic piRNAs along with the detection of other persistent arbovirus small RNAs in the pattern plots (right). The x-axis gives the coordinates of the virus sequence; the y-axis is the autoscaled read frequency. The total small RNA normalized counts are below each plot.
