Nuclear tRNA sequences in the four mel-complex species. (A) The subset of all nuclear tRNAs that differ in copy-number, isotype identity, or anticodon sequence between four mel-complex species. Each box represents an individual tRNA gene copy located within a larger syntenic cluster of tRNAs (grouped together as colored columns). Thick black outlines show tRNAs predicted to be pseudogenes. The thick white outline shows an arginine tRNA on Chromosome 2R predicted to use a different anticodon. (B) Secondary structure alignments of orthologous nuclear tRNAs that show anticodon shifts. The tRNA anticodon (red box), acceptor stem (purple), D arm (red), anticodon arm (green), and T arm (blue) are highlighted in the alignments. See Supplemental Figure S24 for relative position on the chromosomes.
