Immunoglobulin RGM parameters are unique across human individuals. (Inset) For samples from a cohort of 99 unrelated individuals, two kinds of distance were computed: the normalized JSD between RGMPs inferred from these samples and the number of differing IGHV alleles. Additionally, for each sample, we computed the normalized JSD between its own RGMPs and RGMPs inferred from its data replicate. (A) The distribution of the pairwise normalized JSD for 99 individuals of the HUMAN3 data set was computed for subsamples of 1000, 3000, 10,000, and 30,000 sequencing reads. The blue line corresponds to the average distance between data replicates. (B) Heatmap visualization of A for the subsample size of 30,000 sequencing reads: The values on the diagonal correspond to the average distance between data replicates. (C) The number of IGHV gene alleles that differ between any two individuals as a function of the normalized JSD between their RGMP inferred from subsamples of 30,000 sequencing reads.
