Enrichment of Class II proteins at TSRs follows the direction of RNAPII transcription. (A) SET26 is used as a representative protein of Class II TSR-associated factors. Comparison of SET26 ChIP-seq data with strand-specific RNA-seq data (Naguleswaran et al. 2018) shows that SET26 reads are enriched in the same direction as RNAPII transcript reads. Heatmaps show from top to bottom: minus strand reads from unidirectional TSRs (top), plus and minus strand reads from bidirectional TSRs or from two adjacent unidirectional TSRs (middle), and plus strand reads from unidirectional TSRs (bottom). (B) Examples from the different heatmap regions described in A. A single replicate is shown for each protein. Tracks are scaled separately as reads per million (values shown at the end of each track).
