Cellular localization of chromatin-associated T. brucei candidate proteins. The indicated YFP-tagged proteins expressed in bloodstream Lister 427 cells from their endogenous genomic loci were detected with an anti-GFP primary antibody and an Alexa Fluor 568–labeled secondary antibody (red). Nuclear and kinetoplast (mitochondrial) DNA were stained with DAPI (green). Staining of untagged 427 parasites serves as a negative control. Representative images are shown for those candidate proteins that gave a specific ChIP-seq signal. The images are ordered according to ChIP-seq patterns shown in Figure 2A and Figure 5A. Images for all other tagged proteins are included in Supplemental Figure S2. Scale bar, 5 µm.
