Selective expression of peripheral SFs and exons in mTEC. (A) Expression of a set of tissue-restricted (tau > 0.5) genes that encode for bona fide SFs (Supplemental Table 8) in mTEC populations, skin epithelia, cTEC, and peripheral tissues (mouse ENCODE Project). The bars (left) depict the fraction of a set of single mature mTEC that express each factor. Significant differences in expression between immature versus mature (Aire-KO) mTEC or Aire-KO versus Aire-positive mTEC are indicated by red and blue asterisks, respectively (BH adjusted P-value < 0.05, |fc| > 2, DESeq2 analysis of the population RNA sequencing data, n = 2 biological replicates/condition). (B) Patterns of protein-coding exon (>50 bp in length) inclusion for exons with a low inclusion rate in mTEC (mean PSI < 0.1; max PSI < 0.2) that were included in at least one of the peripheral tissues (PSI > 0.5). (C) Microexon (≤30 bp) inclusion in transcripts from protein-coding genes in the mTEC population and peripheral tissue samples (Supplemental Fig. 11B).
