MERINGUE identifies putative cell–cell communication in the cerebellum using Slide-seq data. (A) Adjacency relationship between Slide-seq beads. Orange dots correspond to beads previously annotated as corresponding to the Purkinje layer. Green dots correspond to beads that are spatially adjacent. Gray lines connect each bead with its spatial neighbors and is agnostic to bead density. (B, left) Expression of receptor Ptprz1 in beads annotated to correspond to the Purkinje layer. (Right) Expression of corresponding ligand Ptn in spatially adjacent beads. Same select region highlighted. (C) Bar plot of −log10(adjusted P-value) for the inter-cell-type spatial cross-correlation statistic of all receptors in Purkinje layer beads versus ligands in the spatially adjacent beads. Red line indicates alpha = 0.2 multiple testing corrected significance threshold. (D, left) Expression of ligand Psap in beads annotated to correspond to the Purkinje layer. (Right) Expression of corresponding receptor Gpr37l1 in spatially adjacent beads. Same select region highlighted as D. (E) Bar plot of −log10(adjusted P-value) for the inter-cell-type spatial cross-correlation statistic of all ligands in Purkinje layer beads versus receptors in the spatially adjacent beads.
