Application of MERINGUE to 2D spatial transcriptomic data of the main olfactory bulb (MOB) and 3D-aligned ISH data of the Drosophila melanogaster embryo. (A) Spatially unaware single-cell clustering analysis identifies five transcriptionally distinct clusters corresponding to various known cell layers in the MOB. Spatial spots are colored based on their inferred cell layer annotation. (B) MERINGUE identifies genes with significantly spatially heterogeneous expression in the MOB. Select genes are shown. (C) MERINGUE groups genes with significantly spatially heterogenous expression in the MOB into five primary spatial patterns. Select patterns are shown. (D) MERINGUE's adjacency weight matrix visualized for aligned 3D in situ hybridization data of the D. melanogaster embryo. Each point is an aligned cell. Cells are connected with a red line if they are inferred to be adjacent. A top view and rotated side view are shown. (E) MERINGUE groups genes into spatial patterns in the D. melanogaster embryo. Representative genes from select identified patterns are shown.
