An overview of interactive exploratory analysis pipeline. The integrative and interactive pipeline with several options can be used to analyze the spatial data sets. (A) Spatial data analysis starts with importing and processing raw data sets. The analysis can then be subdivided into image-based analysis (B) and gene expression–based analysis (C). Analysis based on images such as cell segmentation and morphological quantification is available to investigate the cellular intricacies in a selected section of a tissue. Gene expression–based analysis consists of several approaches such as clustering, spatial network construction, and cell type enrichment to visualize gene expression patterns. An interactive graphical interface makes these methods easier accessible for novice users.
