Figure 5.
Testing i-GONAD for generation of conditional alleles using C57BL/6J mice. (A) Plagl1 genotyping results of E12.5 embryos. For the 5′ gRNA region, WT band is 278 bp, loxP band is 312 bp; for the 3′ gRNA region, WT band is 200 bp, loxP band is 234 bp. (B) Clcf1 genotyping results for E12.5 embryos. For the 5′ gRNA region, WT band is 355 bp, loxP band is 389 bp; for the 3′ gRNA region, WT band is 188 bp, loxP band is 222 bp. (C) Summary of bacteria colony genotyping results shown in Supplemental Figure S10C,D.
