Sample collection, sequencing, and alignment pipeline and statistics overview. (A) Biological data sample collection, sequencing, and alignment workflow for SKBR3 breast cancer cell line and 3D Matrigel-grown organoids for solid breast cancer tumor tissues obtained from two females, patient 51 and patient 48. (B) Yield and alignment coverage statistics for observed samples across WGS experiments various sequencing platforms. Suffixes T and N next to patient identifiers indicate tumor or matching normal tissue. Alignment values x (y) represent average read-depth x for aligned reads with (y) representing average read depth when all unresolved Ns in the reference are also taken into consideration. (C) Length distribution for reads of length 1.5+ kbp from PacBio and ONT sequencing runs for patient 51: (raw-yield) lengths of raw sequenced reads; (raw-aligned) lengths of raw reads that had any alignment inferred for them; (aligned) lengths of aligned parts of sequenced reads.
