Cell precision and true positive rate of RNA-Bloom's reference-free and reference-guided modes over 384 cells. The simulated data set of 384 cells is split into four smaller subpools of 96 cells and 1 cell. Pool size of 1 refers to no pooling between cells. Each subpool is assembled separately, and the cell precision of the assembled isoforms in each cell is calculated based on the I95. True positive rate was calculated based on I95, denoted as TPR95. Distributions of cell precision and TPR95 were measured over all 384 cells. The comparison bars on top between different pool sizes indicate statistical significance of the difference between distributions at P < 0.001 (***), P < 0.01 (**), P < 0.05 (*), or no significance (NS) using the Wilcoxon test.
