Figure 4.
Overexpression of MYB125 activates the expression of genes in the lignin biosynthetic pathway. Relative expression levels of (A) C4H1; (B) CSE; (C) F5H; (D) C3′H; (E) PAL2; (F) CCoAOMT1; (G) PAL4; (H) COMT1; and (I) 4CL3. Relative transcript levels were quantified by RT-qPCR and normalized with the housekeeping gene Actin2. (J) Acetyl bromide lignin content of WT and transgenic roots overexpressing MYB125. Error bars, SD. One-tail Student's t-test was used to determine statistical significance; n = 3. Significance is indicated by asterisks: (*) P-value < 0.05; (**) P-value < 0.01.
