ADAR-deficiency perturbs the global splicing landscape in mouse tissues

ADAR can impact splicing in an editing-independent manner. (A) Venn diagram comparing DEXSeq output of Adar^−/− cortex and editing-deficient Adar^E861/E861A brain. (B) Table of DEXSeq events common between Adar^−/− cortex and Adar^E861/E861A brain. Genes with opposite trends in the direction of change are highlighted in yellow. Log₂FC > 0 indicates higher quantity in WT and Log₂FC < 0 indicates higher quantity in knockout. (C) Histogram showing qPCR validation of splicing events in IFIH1-rescued MEFs. Data shown are mean inclusion to exclusion ratio in Adar^−/− and Adar^E861A/E861A (±SD) relative to Adar^+/+. Statistical test performed was Student's t-test; (*) P < 0.05, (**) P < 0.01, (***) P < 0.001.