Differential miRNA expression analysis. (A) Differential expression analysis of miRNAs using FANTOM5 sRNA sequencing data in aortic smooth muscle cells in human compared to mouse, rat, dog, or chicken. The red and blue bars correspond to the percentage of expressed orthologous miRNAs with significantly (Benjamini–Hochberg corrected P < 0.1) higher and lower expression, respectively, in human compared to mouse, rat, dog, or chicken. The number N of expressed orthologous miRNAs in each comparison is shown on the right. (B) Differential expression analysis of miRNAs in human compared to mouse, rat, dog, and chicken; using CAGE expression of the pri-miRNA as a proxy for the expression level of the mature miRNA. The red and blue bars correspond to the percentage of expressed orthologous miRNAs with significantly (Benjamini–Hochberg corrected P < 0.1) higher and lower expression, respectively, in human compared to mouse, rat, dog, or chicken. The number N of expressed orthologous miRNAs in each comparison is shown on the right. (C) Percentage of miRNAs differentially expressed in each comparison, separately based on the evolutionary age of each miRNA. The one-sided P-value of a Poisson regression model against the evolutionary age category is shown on the right, together with the number N of expressed orthologous miRNAs in each comparison. (D) Percentage of miRNAs differentially expressed in each comparison, separately based on the evolutionary age of each miRNA; using CAGE expression of the pri-miRNA as a proxy for the expression level of the mature miRNA. The one-sided P-value of a Poisson regression model against the evolutionary age category is shown on the right, together with the number N of expressed orthologous miRNAs in each comparison.
