Positioning the mRNA tunnel within ribosomes using RIC/WCE-ratios. (A) Distribution of ribosomal proteins (RPs) in the normalized poly(A)+ interactome. Volcano plot of noCL-normalized (left) or WCE-normalized (right) RIC as in Figure 1D. Proteins annotated as cellular component “cytosolic ribosome” [GO:0022626] are designated in orange, with the molecular function “RNA binding” [GO:0003723] in black. For proteins annotated with both GO terms, GO:0022626 was given precedence. The RPs that were most significantly enriched on poly(A)+ RNA are indicated in blue. Proteins without WCE signal were disregarded (see Supplemental Figs. S1D, S2A). For reference, standard gene names for enriched RPs are given in Supplemental Fig. S2B. (B) Structure of the Saccharomyces cerevisiae 80S ribosome [PDB:5MC6] (Schmidt et al. 2016). RPs with S. pombe orthologs that were highly significantly enriched on poly(A)+ RNA are designated in blue. For reference, the mRNA is colored black, tRNAs bound in the ribosomal A- and P-sites in red, and the small and large ribosomal subunits in beige and white, respectively.
