Comparison of human STRIPE-seq to CAGE, RAMPAGE, and nanoCAGE-XL. (A) Hierarchically clustered heatmap of Spearman's ρ values for pairwise comparisons of STRIPE-seq, CAGE, RAMPAGE, and nanoCAGE-XL signal within promoter regions (−500 to +500 bp relative to an annotated TSSs). Before clustering, samples were thresholded such that each promoter had to have at least 3 read counts in one sample, and then counts were TMM normalized. (B) Genome browser-style tracks showing CPM-normalized STRIPE-seq, CAGE, RAMPAGE, nanoCAGE-XL (replicate 1 for each input amount), and poly(A)+ RNA-seq (replicate 1) at a representative region of the human genome. (C) Dinucleotide frequencies at TSSs in all replicates of all technologies. (D) Jitter plot of the number of transcripts with a promoter-proximal TSR in each sample. Error bars represent standard deviation.
