A long-read RNA-seq approach to identify novel transcripts of very large genes

Phasing and quantifying additional titin transcript structures using exPhaser. Exons are numbered by NM_011652 (N2-A) unless noted. (A) Focused phasing of skeletal muscle N2-A isoform defining exons (47, 48, 49, 50) and one cardiac N2-B exon (45*). (B) Focused phasing of cardiac N2-B isoform defining exons (45, 46, 168, 169) and two N2-A defining exons (47 and 167). (C) Phasing of exon 312, a cassette exon only spliced out of skeletal muscle transcripts. (D) Phasing of exon 45^‡ of ENSMUST00000099980, an alternate 3′ terminal exon. (E) Phasing of exons 11–13. The most abundant isoforms are unannotated. Transcript structures listed with multiple accession numbers cannot be uniquely assigned using the exons provided.