A long-read RNA-seq approach to identify novel transcripts of very large genes

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Figure 6.
Figure 6.

Identifying and quantifying Nrap, titin, and nebulin transcript isoforms using exPhaser. Exons highlighted as red squares were validated using RT-PCR and Sanger sequencing. (A) Nrap transcript structures. Exon numbering based on NM_008733. (B) Titin transcript structures. Exon numbering based on NM_011652. The bottom splice pattern could not be uniquely mapped to a single annotation. (C) Nebulin transcript structures. Exon numbering based on NM_010889. Some neighboring constitutively expressed exons were also included for clarity.

This Article

  1. Genome Res. 30: 885-897

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