Coexpression enrichment analysis at the single-cell level reveals convergent defects in neural progenitor cells and their cell-type transitions in neurodevelopmental disorders

Coexpression enrichment analysis of high-confidence NDD genes in six major cell types. (A) Coexpression fold enrichment of eight NDD gene sets (four with dnLoF mutations and four with dnMis mutations) in six cortical cell types, along with the sample size of each. Gene set size is shown in parentheses. Circle size is proportional to enrichment score. (B,C) Coexpression fold enrichment of dnLoF-ASD (B) and dnMis-Epi genes (C) in six major cell types by downsampling the same number of cells for each cell type. Violin plot shows the mean value (point). P-value indicates whether the mean coexpression fold enrichment score of the corresponding gene set is higher than that of the background genes (one-sided Fisher's exact test). (D,E) Coexpression networks of dnLoF-ASD (D) and dnMis-Epi genes (E) in the six cell types using the original sample size. Node size is proportional to coexpression degree.