Deconvolution of bulk human-specific expression differences using neuronal evolutionary signature. (A) Overlap of human-specific genes, defined as those showing twofold greater human-macaque expression difference relative to the chimpanzee-macaque and bonobo-macaque difference, between bulk RNA-seq and snRNA-seq data sets for genes preferentially expressed in a specific neuronal subtype (Supplemental Table S5). Colors indicate brain regions. The x-axis labels indicate neuronal subtypes. (B) Percentages of genes showing human-specific expression in each brain region in bulk RNA-seq data set overlapping with genes showing human-specific expression in neuronal subtypes in snRNA-seq data. Empty circles indicate the three brain regions used in the snRNA-seq experiment. The dashed line represents an average overlap for three brain regions used in the snRNA-seq experiment. (C) Anatomical localization of 33 regions in the human brain colored according to the overlap between human-specific expression differences in bulk RNA-seq and in neuronal subtypes.
