CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs

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Figure 4.
Figure 4.

Single-base editing in the PAM-proximal region using target-mismatched sgRNAs. (A) Negative selection of edited target sequence (C578A in the galK gene) by Cas9/target-mismatched sgRNA complexes. Single or double mismatch(es) were located adjacent to the C578A point mutation site. (B) Genome editing efficiencies using target-matched, single- or double-base mismatched sgRNAs and the number of surviving cells. Each bar represents the mean of three independent experiments.

This Article

  1. Published in Advance April 23, 2020, doi: 10.1101/gr.257493.119 Genome Res. 30: 768-775

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