Figure 3.
Negative selection of single-base-edited DNA targets aided by target-mismatched sgRNAs via CRISPR-Cas9. (A) Mismatch tolerance allows unedited targets to be cleaved by the Cas9/target–mismatched sgRNA complex. Single-base-edited targets cannot be recognized by the Cas9/target–mismatched sgRNA complex owing to multiple mismatches. (B) Design of target-mismatched sgRNAs for single-base editing (T504A) in galK. Single or double mismatch(es) were located adjacent to the T504A point mutation site. (C) Single-base genome editing efficiencies using single- or double-base mismatched sgRNAs and the number of surviving cells. Each bar represents the mean of three independent experiments.
