Figure 2.
Design and construction of the CRISPR sgRNA library for B. mori cells. (A) Flowchart for constructing the CRISPR sgRNA library for B. mori cells. (B) Design for all sgRNAs in all B. mori gene exons (lower), and the sgRNAs selected for the subsequent experiment (upper); see Supplemental Table S2. (C) Second-generation sequence analysis of the sgRNA distribution for the pB-CRISPR library (orange) and BmEGCKLib (green). (D) Pie chart showing the percent distribution of the sgRNAs. (E) Overlap in sgRNA distribution between the pB-CRISPR library and BmEGCKLib. (F) Correlation analysis between the pB-CRISPR library and BmEGCKLib.
