Treatment with phenanthroline (PH) leads to rapid loss of Pol II and transcription-associated proteins. (A) Differential and dynamic binding behavior of proteins representing different chromatin processes upon treatment with PH and AU. The lines indicate the three different BC pairs of the indicated TAP-tagged proteins in the Chrom-3×BC library (Log2 IP/input at time points indicated in Fig. 2). (B) Zoom-in of heatmap of Figure 2 showing the Pol II subunits present in the library. (C,D) Immunoblot analysis of the largest subunit of Pol II (Rpo21-TAP) with and without PH and AU treatment in G1-arrested cells at 16°C. Pgk1, Hmo1, and a nonspecific band (*) were used as loading controls. (E) ChIP-qPCR analysis of Rpo21 binding at the BC_UP and BC_DN regions in G1-arrested cells treated with (15 and 60 min) and without (Pre) PH at 16°C (average of three biological replicates ± SD).
