Retrospective lineage tracing accuracy based on microsatellite capture of single cells from the HCT116 ex vivo tree. (A) Diagram of complete phylogenetic tree with colors corresponding to clones from the original ex vivo tree. (B) Accuracy of tree calling, as determined by the rate of topologically correct triplets reconstructed, based on the varying number of estimated cell division differences between each clone. An overall tree accuracy of 88% was observed by using all possible triplets from the tree. (C) Overall tree accuracy as a function of maximum cell divisions recapitulated in the ex vivo tree compared to existing methods for phylogenetic tree mapping. Arrow indicates optimal desired results of maximizing accuracy at lower cell division differences.
