Figure 1.
Overview of RETrace Method. (A) Single-cell processing includes FACS sorting single cell per well in a plate, followed by restriction enzyme digestion, ligation of methylated hairpin loop adapters, and single-primer PCR for linear amplification. (B) Methylation postprocessing with bisulfite conversion followed by PCR utilizing primers specific to the MspI cut site, which results in cell type identification. (C) Microsatellite postprocessing with hybridization probe enrichment, which results in building a phylogenetic tree.
