STAG1 and STAG2 are important in the regulation of genes involved in neuronal development. Genes found to be involved in neuronal development and misregulated by STAG1 and STAG2 degradation were tested in neural stem cells under control (Ctrl), STAG1 (shSTAG1), STAG2 (shSTAG2), and SMC1A (shSMC1A) siRNA knockdown to test whether they depend specifically on STAG1 or STAG2 or on the presence of the cohesin complex in general. The tested genes are: CDK6, associated with microcephaly (Faheem et al. 2015); AXL, intellectual disability (Burstyn-Cohen 2017); IL6ST, neural protection and development (März et al. 1997); ADAM19, neurogenesis (Alfandari and Taneyhill 2018); GAL, neuro-regulatory peptide-encoding gene (Borroto-Escuela et al. 2017). Mean of n = 3; t-test P-values are indicated. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001.
