The MAC chromosomes are related to the MIC TAD-like structures during conjugation. (A) The heatmap regions (Chromosome 1: 3.2 Mb to 7.2 Mb) of the MIC (Smc1-HA HiChIP at 3 hpm, upper) and the MAC (whole cells Hi-C at 3 hpm, below) compared to the CBS sites on the MIC and the MAC scaffolds are plotted. (B) The heatmap showing the Hi-C interactions on six of the MAC scaffolds at 3 hpm (whole cells Hi-C data mapped to the MAC scaffolds). (C) The heatmap showing the Hi-C interactions on the MIC Chromosome 4 (4.11 Mb to 8.14 Mb) at 3 hpm (whole cells Hi-C data mapped to the MIC genome). (D) The heatmap showing the Hi-C interactions on the MIC Chromosome 4 (4.11 Mb to 8.14 Mb) at 3 hpm (Smc1-HA HiChIP data mapped to the MIC genome). (E) A model for the MAC chromosomes development during the conjugation of T. thermophila. The TAD-like structures established in the zygotic nuclei were followed by chromosome breakage at CBS sites.
