APA-mediated loss of tumor-suppressive miRNA binding sites is associated with poor patient outcome. (A) Number of genes that lose highly conserved miRNA binding sites because of 3′-UTR shortening. The percentage of genes that lose at least one miRNA binding site is indicated above the bracket. (B) Highly conserved miRNA families were plotted against their Z-score, an index of the lost binding sites in which a more negative Z-score indicates more significant binding site loss. (C) t-SNE plot depicting TCGA patient clusters in the highly conserved miRNA feature space. (D) Kaplan–Meier survival plot for the three patient clusters identified in C: (*) P < 0.05 for Cluster 1 to Cluster 3 comparison. (E) Heatmap depicting the association of miRNA binding site loss (miR score) with patient clusters.
