Capture of annotated and novel full-length splice isoforms. (A) Number of gene and isoform captures with full-length support in our data set (left) versus the WormBase (WB) annotation (right) (Lee et al. 2018; WormBase web site [https://wormbase.org], release WS265 2018). (B) Stacked bar graph showing overlap between isoforms and genes with full-length support in our data set and those with full-length support in the WormBase annotation. (C) Number of previously annotated splice isoforms and corresponding genes identified by our data across all stages. (D) Density plot showing the number of isoforms identified per gene across our full data set and the WormBase annotation. (E) Number of novel isoforms and genes with novel isoforms identified across all stages. (F) Density plot showing the proportion of novel splice isoforms with a given number of reads supporting their structure.
