Statistics of the newly called isoforms with long reads. (A) Summary of the isoforms called using long reads. Left: Bar plots of the number of existing isoforms that are recovered (defined as coverage of over 95% length of an existing transcript by at least a single long read, colored in black) or uncovered (the remaining isoforms, colored in gray). Right: Bar plots of the number of novel isoforms called by TrackCluster. Breakdown of the novel isoforms is color-coded as in Figure 2 and is also shown at the bottom. (B,C) Abundance (B) or length distribution (C) of the novel isoforms of various categories as defined in Figure 2. (D) Count of the isoforms output by TrackCluster (gray), existing isoforms (yellow), and their ratio (blue) over read length, in nt. (E) An example of TrackCluster predicted isoforms. Shaded in blue is the accumulative long read coverage of unc-52 in all developmental stages. Shaded in gray are three novel isoforms (colored in red or green as in B) supported by the highest read coverage along with the existing isoforms (black) to which they bear the highest similarity. Genomic coordinates are shown at the top, in kb.
