Fraction of full-length RNA reads (defined as reads with an SL signal or covering ≥95% length [indicated by dashed line] of an annotated transcript) out of all RNA reads. (A) Count distribution of reads over the fraction of the mapped length out of the length of existing transcripts (WS260). (B) Proportion of reads over the fraction of their mapped lengths against those of the annotated transcripts derived from embryos (EMB), larvae (L1), and young adults (YA). (C) Distributions of read count over read length. The reads with and without SL and the simulated reads are colored in brown, gray, and blue, respectively. The annotation simulation curve was generated by replacing the actual length of long reads with the length of an annotated transcript to which it shows the highest similarity. (D) Distribution of full-length reads derived from the mitochondrial genome. Top: Read coverages of mitochondrial genes color-coded based on the respiratory chain complex. Bottom: Fractions of full-length reads for individual genes. Read counts are shown proportional to the circle area.
