Experimental design and bioinformatic pipeline to identify sex-linked genes. (A,B) The SEX-DETector analysis relies on obtaining genotyping data from a cross (parents + F1 progeny). (C) SEX-DETector infers the segregation type based on how alleles are transmitted from parents to offspring. Three segregation types are included: autosomal (alleles of the parents are transmitted to the progeny the same way in both sexes, in a Mendelian way), XY (one allele of the father—the Y allele—is transmitted exclusively to sons), X-hemizygous (the single allele of the father is transmitted exclusively to daughters; the sons get one allele from the mother only). See Methods for more information. C. sativa male and female plants pencil illustration by annarepp/Shutterstock.com.
