Features associated with alternative exons and tissue-regulated alternative exons. For panels A–E, comparisons were made between constitutively spliced exons (pink), alternative exons (light blue), and tissue-regulated alternative exons (gold). (A) Comparison of frequency of frame preservation (percentage of exons that are a multiple of 3 nt). (B) Frequency of exons overlapping with a UniProt conserved protein domains. (C) The frequency of exons overlapping with predicted disordered regions in proteins. (D) Distributions of the lengths of introns flanking internal exons from same comparison groups. (E) Distribution of the lengths of exons. (A,C) Pearson's chi-squared test: (*) P ≤ 0.01; (**) P < 1 × 10−3; (***) P < 1 × 10−10. (D,E) Wilcoxon rank-sum test: (*) P ≤ 0.05; (**) P < 1 × 10−15.
