Figure 5.
Coamplification of testis-specific gene families on X and Y Chromosomes. (A) Extended metaphase and interphase FISH analysis using CHORI-240 BAC probes containing HSFY (red, C0365D18) or HSFX (red, C0054K01). (B) Phylogenetic analysis of HSFY and HSFX amino acid sequences in mammals; chicken autosomal homolog was used as an outgroup. Branch lengths are proportional to substitution rates. Numbers at nodes indicate support from 100 bootstrap replicates. Gray bars highlight multicopy gene families within species. For bull, only 11 of 79 nearly identical HSFY copies for which we have sequence were included in alignment; all 11 copies of HSFX for which we have sequence were included.
