Conservation of major MSY repeats across bovine species. Two-color FISH analyses in five different Bos taurus breeds, two Bos indicus breeds, and seven wild bovine species. (A) Positions of CHORI-240 BAC FISH probes within sequenced bull MSY. Five different probe combinations were used. In all experiments, the red probe is the same BAC, derived from the long-arm amplicon. The green probe differs among experiments, as indicated. (B) Phylogenetic tree, drawn to scale, represents evolutionary relationships among bovine species. Representative Y Chromosomes observed in extended metaphase FISH images are shown for each experiment in each species. Red and green signals derive from colored FISH probes diagrammed in A.
