SINEs are up-regulated by activity in dentate granule (DG) cells. (A) Study design from Jaeger et al. (2018), in which mice were initially raised in a home-cage (HC) environment with minimal stimulation. They were then exposed to a novel environment (NE) for 15 min, which was sufficient to activate the IEG response. They were returned to the HC for 1 h to facilitate sufficient elevation of IEG expression. Nuclei were extracted, sorted on FOS, and prepared for snRNA-seq. (B) T-SNE of FOS+ (red) and FOS− (blue) nuclei from either VIP, CA1, or DG neurons. (C) log-Fold change (logFC) of all SINEs detected in DG, CA1, or VIP nuclei. Black = SINEs differentially expressed between FOS+ versus FOS− neurons after multiple-testing correction. (D) B2_Mm1a and B2_Mm1t expression in VIP, CA1, or DG neurons in FOS− or FOS+ nuclei. Boxes indicate upper, middle, and lower quartile range. Dots indicate measurements beyond 1.5 times the interquartile range.
