Agonist-induced functional analysis and cell sorting associated with single-cell transcriptomics characterizes cell subtypes in normal and pathological brain

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Figure 2.
Figure 2.

Gating-dependent cell populations. (A) Discrimination of cells based on scatter parameters: (FSC) forward scatter; (SSC) side scatter. Cells were gated according to their size/structure. (GD1) Gating-dependent cell population 1; (GD2) gating-dependent cell population 2. (B) RNA was purified from WT GD1 and GD2 cells. Neuronal and nonneuronal marker levels were analyzed in both populations by RT-qPCR. The graph (on the left) shows mRNA expression relative to the total WT neuron suspension (WT input). GD1 and GD2 cells were live-imaged 2 h after aiFACS sorting (on the right). 63× magnification; scale bars, 15 μm.

This Article

  1. Published in Advance September 24, 2020, doi: 10.1101/gr.262717.120 Genome Res. 30: 1633-1642

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