An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation

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Figure 1.
Figure 1.

Optogenetic control of Set2 subcellular localization. (A) Schematic of histone H3 lysine 36 (H3K36) methylation triggered by light-induced translocation of LANS-Set2 into the nucleus as well as demethylation by Rph1. (B) Confocal images from Supplemental Movie S1 demonstrating reversible control of mVenus-tagged LANS-Set2 localization in yeast cells with histone H2B endogenously tagged with mCherry (scale bar, 3 μm). (C) Quantification of nuclear/cytoplasmic fluorescence intensity change before, during, and after light activation. Mean ± SEM was calculated from the activation of multiple cells (n = 3) shown in B and Supplemental Movie S1.

This Article

  1. Published in Advance October 5, 2020, doi: 10.1101/gr.264283.120 Genome Res. 30: 1605-1617

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